The following information must be provided on each test: Demonstrated by cytopathogenic effect, flourescent antibody, plaque count, animal response, or other recognized acceptable technique Note: T - Toxic, + - Virus recovered, 0 - No virus recovered. (Total of 120 replicates or carriers for each sample). Section 4 of the Pest Control Products Act requires that antimicrobial products imported into or sold in Canada must be registered. General protocols, propagation of cultures, fabric to water ratios, test materials, water temperatures and subculture media must be the same as that specified in the tentative method. The contents of the Federal Register are required to be judicially noticed (44 U.S.C. Administration. Germicidal and Detergent Sanitizers Test, Certification that all materials and devices making up the container are identical to those specified by the basic manufacturer. Prepare culture suspensions as per section 4.026 (Methods AOACFootnote 2). The following protocol may be utilized: Three product samples representing three different preparations must be tested against each test bacterium on at least five replicates of each representative surface for which the product is intended for use. The squares should be dried for 20 to 30 minutes in a bacteriological incubator at 30 to 37 C. A "zero time" bacterial numbers recovery test must be performed to show the efficiency of the recovery process and must be reported. Do not house livestock or employ equipment until treatment has been absorbed, set or dried. Residual bacteriostatic claims for laundry additives must be substantiated with efficacy data derived from the AOAC Bacteriostatic Activity of Laundry Additives Method (Test D.02.010R072). Foil will serve to prevent contamination of controls during spraying. subtilis and. notify members of the meetings; and (2) Acts in an advisory capacity on all matters pertaining to health and safety for the guidance of the employer and the workers. It is used in the production of acrylic fibers, resins and rubber. The book will be of value to all those engaged in the manufacture of dipped-latex products, production plant supervisors, those working in quality control, testing laboratories and technical support functions. b. report status of recommendations made; c. d. submit to the employer a report of the activities of the committee, including recommendations made. The 60-day shelf-life study should be carried out with two test surfaces against one test bacterium. Fungicidal Test on duplicate samples of one preparation. Three samples representing three product preparations are tested, with five 1-inch square treated swatches against S. aureus and Klebsiella pneumoniae, aberrant, ATCC 4352 on seeded agar for each sample. The squares should be dried for 20 to 30 minutes in a bacteriological incubator at 30 to 37 C. A "zero time" bacterial numbers recovery test must be performed to show the efficiency of the recovery process and must be reported. All carpet samples tested must be fully identified, and the pile fiber type, pile yarn weight of finished carpet, pile density and tuft height reported. The chemical industry comprises the companies that produce industrial chemicals.Central to the modern world economy, it converts raw materials (oil, natural gas, air, water, metals, and minerals) into more than 70,000 different products.The plastics industry contains some overlap, as some chemical companies produce plastics as well as chemicals. CDC Science Clips: Volume 10, Issue 19, May 29, 2018. Office of the Federal Register. The following list of tests (Table 1) are used to support the claims for various disinfectant-type products. The "zero time" test should show the loss in viability that occurred during the drying. Representative test surfaces may include, but are not limited to glass, metal, unglazed or glazed ceramic tile, vistreous china, or wooden flooring depending on the uses proposed on the labels. Note: It should be understood that where test organisms other than those specified in the Methods outlined above are substituted therein, the media, incubation time, and other factors will all be evaluated in terms of the significance of the results claimed. A sufficient volume of testing so that deficiciencies which may be subsequently found in the Laboratory Services Division cannot be ascribed to variations in testing which may be encountered among different laboratories. The book will be of value to all those engaged in the manufacture of dipped-latex products, production plant supervisors, those working in quality control, testing laboratories and technical support functions. Manual of Methods. States may fulfil their obligation under this paragraph by a declaration of self-insurance. 1970. Germicidal Spray Test (for spray surface disinfectants). If not, the label must bear a disclaimer for such use. Scrub the 3 wet spots in the same manner as test carpet. In addition, one sample preparation representing a 60-day shelf-life stability study must be tested in duplicate using one test bacterium. Chemical analysis may be substituted for biological testing for organic chlorine-bearing chemicals. 1969. Since most germicidal chemicals are inherently toxic for the host systems employed in virological techniques (especially cell cultures), either a "detoxification" procedure which will render the treated virus free of residual germicide, or a virus concentration of sufficient titer to demonstrate at least a three-log reduction even when cytotoxicity is observed in tissue culture systems must be employed in the study. Liquid" means a substance or mixture of substances which is capable of a visually detectable flow as determined under ASTM D-4359-90 (2006), incorporated by reference in 2.8.3 of this regulation. A 99.9% reduction of test bacteria over the control count must be demonstrated in each case. In order to simulate in-use conditions, the specific virus to be tested must be inoculated onto hard surfaces, allowed to dry, and then treated with the product according to the directions for use on the product label. Sanitizing rinse claims for iodophors, mixed halides and chlorine-bearing chemicals must be substantiated with efficacy data derived by the Available Chlorine Germicidal Equivalent Concentration Method (Test D-03.020R-70). Exposure time intervals between zero time and ten minutes should be tested for the product as well as for the untreated controls. Notwithstanding the requirements of 2.5.2 of this regulation, lawn and garden insecticides (aerosol) may claim to kill insects and other arthropods. " Data from duplicate samples of one or these preparations, representing a 60-day shelf-life stability study, showing concentrations of the product equivalent in activity to the three reference standards must be furnished. Tests may be carried out in a washing machine in lieu of the Petrocci and Clarke method. To substantiate the sanitizing claims for a product, the applicant must submit data prior to registration to show that the product, when used as directed, will substantially reduce the numbers of test microorganisms on a surface over that afforded on an untreated control surface. Official Methods of Analysis of the Association of Official Analytical Chemists' 11th Edition, 1970. Tuberculocidal Test on one preparation (one sample). A.O.A.C. Determine the count of bacteria in an 18 to 24-hour culture and add a 0.01 to 0.03 ml. Synonyms for acrylonitrile include acrylon, carbacryl, cyanoethylene, fumigrain, 2-propenenitrile, VCN, ventox and vinyl cyanide. B.S. 18, 2001 CODE OF FEDERAL REGULATIONS 29 Part 1910 (§ 1910.1000 to End) Revised as of July 1, 2001 Labor Containing a codification of documents of general applicability and future effect As of July 1, 2001 With Ancillaries. Duplicate samples of one preparation representing 60-day shelf-life stability must be tested with five replicate fabric swatches on agar seeded with S. aureus. Acrylonitrile Acrylonitrile is included in this report as it can be used to manufacture acrylic acid and is commercially used to produce acrylamide. With products proposed for registration that duplicate a formulation which is registered but not intended to be marketed by a basic manufacturer, the data developed and on file may be applied provided: Products proposed for registration that are identical to products already registered, manufactured and marketed by a basic registrant may be accepted provided: The basic efficacy data developed by the supplier of the registered concentrate may be utilized under the following guidelines: Prepare 8 ounce French square culture bottles with NUTRIENT AGAR B (Methods AOACFootnote 2, 4.023 (a), (2)) composed of 3 grams beef extract, 5 grams Anatone and 30 grams agar per liter. Smoking tobacco and cigarettes is another potential exposure. Testing aerosol products for germicidal and sanitizing activity. Until an official method has been adopted, the Division will accept adequate data developed by any virological technique which is recognized as technically sound. Exemptions to this requirement are listed in Section 3 of the Regulations under the Pest Control Products Act and are described in Trade Memorandum T-1-214. Use of any of these products could lead to the exposure to acrylonitrile. Four bound copies of all submitted information should be sent to the following address: Pesticides Section Plant Products and Quarantine Division Agriculture Canada Ottawa, Ontario K1A 0C6, S.W. China to drive global propylene industry growth between 2019 and 2030, says GlobalData. Claims against specific pathogenic fungi must be supported with efficacy data derived from duplicate samples of one preparation using the AOAC Fungicidal Test. General disinfection claims are much broader than minimal claims and must be substantiated with the efficacy data required for minimal claims, along with efficacy data derived from two other test species. Feb. 8, 2013 Title 29 Labor Part 1910 (§ 1910.1000 to end of part 1910) Revised as of July 1, 2013 Containing a codification of documents of general applicability and future effect As of July 1, 2013. Note: The scrubbing procedure should be conducted in a biological hood or in a glove box. HOW TO USE THE CODE OF FEDERAL REGULATIONS Benzene is a colorless, flammable liquid with a sweet odor. Enter the email address you signed up with and we'll email you a reset link. Methods prescribed by the American Association of Textile Colorists and Chemists involving agar plates (see Test D-02.020S-65) or liquid culture media (see Test D-02.030S-65) may be employed to support residual bacteriostatic claims. Benzene is a colorless, flammable liquid with a sweet odor. The added wash water to media volume ratio should not exceed 1:40. The term "antimicrobial product" includes such subcategories as: When labels for antimicrobial products are submitted as requested in the Pest Control Products general "Registration Guidelines", antimicrobial label claims subject to regulation under the Pest Control Products Act must be supported by efficacy data as outlined in the attached "Guidelines for Registering Antimicrobial Products". Adequate controls must demonstrate that bacteriostatic agents in the carpet pile or backing do not interfere with the test results. This replaces Memoranda R-1-53, dated June 17, 1974 and T-1-215, dated February 14, 1980. Benzene is formed from natural processes, such as volcanoes and forest fires, but most exposure to benzene results from human activities. Claims of virucidal activity for a product must be restricted to those viruses which have actually been tested. If the product is a spray, the procedure must be modified to conform with the AOAC Germicidal Spray Product Method (Test D-01.060R-70) using the media, microorganisms, etc., described in the AOAC Tuberculocidal Activity Method. 4. Reports and Publications – Consumer Product Safety, Pesticides and pest management reports and publications. Note: The above directions for use must appear on labeling of products containing cresylic acid, synthetic phenols, pine oils or quaternary ammonium compounds. Acrylamide is imported by WA's flocculant and viscosity modifier manufacturers (see next page) where it represents about 20 per cent by value of raw material inputs (comparable to acrylic acid). We would expect this to be 12 gallons of dilute product to 800 to 1200 square feet of carpet. Group of crude-producing countries which has used its collective weight of production since OPEC was founded in 1960 in an attempt to influence oil prices. ASTM Test Method D-2574-67T. National Archives and Records. 1510). If the proposed product, other than a pressurized spray, is to be manufactured and/or formulated by the applicant, the criteria set forth and marked in enclosure Duplicated Products Formulations must be complied with. RESTRICTIONS ON TRANSPORT. Determine the number of viable organisms in each sample bottle by plating duplicate dilutions in Standard Methods Agar with appropriate neutralizer. 1200 square feet of carpet 30 replicates with one test bacterium the n zero time '' test show... Per test surface using a bacteriological loop Evanston, Illinois not demonstrate measurable reactions... Time intervals between zero time and ten minutes should be taken not to wet or scrub the... Viability that occurred during the drying be registered markets ethylene, its co-products and derivatives new. Available chlorine Germicidal Equivalent Conc the data requirements outlined above for laundry additives test, specified! 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